![]() ![]() Only V2 library preparation is drawn here. Oligo sequences are provided for both V2 and V3 if they are different. The cell barcodes whitelist for the V3 chemistry can be found from the Cell Ranger software (using Cell Ranger v3.1.0 as an example): cellranger-3.1.0/cellranger-cs/3.1.0/lib/python/cellranger/barcodes/. The final libary structure is exactly the same, except that the UMI is 10-bp long in V2 but 12-bp in V3. In terms of the 3' gene expression library preparation, there are only a few nucleotide differences for some oligos. In addition, the oligo beads are modifidied to support feature barcoding. The V3 chemistry gave better sensitivity (detects more genes) comparting to the V2 chemistry. This file is copied from Cell Ranger (using Cell Ranger v2.1.0 as an example) /path/to/cellranger-2.1.0/cellranger-cs/2.1.0/tenkit/lib/python/tenkit/barcodes. You can find out all the cell barcodes (16 bp) here: 737K-august-2016.txt. Oligo sequence information is taken from The 10x Genomics Technical Note. The Chromium Single Cell 3’ Solution V2 chemistry is shown here. 10x Chromium Single Cell 3' Solution V2 and V3 10x Chromium Single Cell 3' Solution V2 and V3
0 Comments
Leave a Reply. |